Adenylate Cyclase (ACT), Recombinant from Bordetella Pertussis
|964-430||> 5 mg||Inquire|
Pertussis toxin is supplied in a buffered solution containing 0.05M Tris-Cl (pH 7.6), 0.5M NaCl, 50% glycerol at desired concentration between 0.1 to 0.5 mg/ml.
Pertussis toxin is purified from cultured supernatants of Bordetella pertussis (Tohama Phase I strain).
Pertussis toxin (PT) is a complex protein composed of five noncovalently linked subunits named S1 (26 kDa), S2 (23 kDa), S3 (22 kDa), S4 (12 kDa), and S5 (11 kDa). The five subunits are assembled into the holotoxin comprising monomer A (subunit S1) and oligomer B (subunits S2, S3, S4, and S5) in a molar ratio of 1:1:1:2:1.
Protein concentration is based on Bradford assay using bovine serum albumin (BSA) as standard.
Biological activity of the purified PT is analyzed by the CHO cell clustering assay. Results indicated that the minimum concentration of PT that induced at least 50% CHO cell clustering and morphology changes was < 5 pg/ml.
Store at -20°C. To insure uniformity, it is recommended that the pertussis toxin suspension be swirled or gently mixed prior to use.
Purity >99% by SDS-PAGE.
Reference: Kapasi A, Pikaytis, Pawloski L, et al. Comparison of different sources of pertussis toxin as coating antigens in IgG Anti-PT ELISAs. Presented at the 9th International Bordetella Symposium, October 2010, Baltimore MD.
Quality control documentation of each lot is provided with shipment.