Lipooligosaccharide (LOS) from Bordetella pertussis (Tohama I strain)
|964-530||> 5 mg||Inquire|
LOS is supplied in pyrogen free water at a desired concentration of 0.1 to 0.5 mg/ml.
Lipopolysaccharide (LPS) is a constituent of the outer membrane of bacteria. The LPS of B. pertussis has a highly branched core structure, no repeating O-side chain, and an unique non-repeating terminal trisaccharide. Due to the lack of O-side chain repeating sugars, the LPS of of B. pertussis is commonly referred to as lipooligosaccharide (LOS). LOS is purified from the cells of Bordetella pertussis (Tohama I strain). After initial extraction of cells with hot phenol, purification consists of proteinase K treatment followed by ultracentrifugation.
Store at -20°C. To insure uniformity, it is recommended that the LOS suspension be thawed on ice and then vortexed for 3-5 min. to counteract any absorption of the LOS to the tube surface. If any aggregation of the LOS is observed, warm suspension for 5-10 min at 55°C and repeat vortexing.
Electrophoresis of B. pertussis LOS reveals two bands, referred to as bands A and B. The principle and slower migrating band A is composed of lipid A, core oligosaccharide, and the terminal trisaccharide. The faster migrating band B contains lipid A and core oligosaccharide, but lacks the terminal trisaccharide.
Protein content of the purified LOS is and nucleic acid content less than 1% (A260 absorbance plus agarose gel electrophoresis).
Quality control documentation of each lot is provided with shipment.