Lipooligosaccharide (LOS)

Lipooligosaccharide (LOS) from Bordetella pertussis (Tohama I strain)

Catolog #

Quantity

Price

964-500 50 μg Inquire
964-510 200 μg Inquire
964-520 1 mg Inquire
964-530 > 5 mg Inquire

 
Contents
LOS is supplied in pyrogen free water at a desired concentration of 0.1 to 0.5 mg/ml.

Description
Lipopolysaccharide (LPS) is a constituent of the outer membrane of bacteria. The LPS of B. pertussis has a highly branched core structure, no repeating O-side chain, and an unique non-repeating terminal trisaccharide. Due to the lack of O-side chain repeating sugars, the LPS of of B. pertussis is commonly referred to as lipooligosaccharide (LOS). LOS is purified from the cells of Bordetella pertussis (Tohama I strain). After initial extraction of cells with hot phenol, purification consists of proteinase K treatment followed by ultracentrifugation.

Storage/Handling
Store at -20°C. To insure uniformity, it is recommended that the LOS suspension be thawed on ice and then vortexed for 3-5 min. to counteract any absorption of the LOS to the tube surface. If any aggregation of the LOS is observed, warm suspension for 5-10 min at 55°C and repeat vortexing.

Purity
Electrophoresis of B. pertussis LOS reveals two bands, referred to as bands A and B. The principle and slower migrating band A is composed of lipid A, core oligosaccharide, and the terminal trisaccharide. The faster migrating band B contains lipid A and core oligosaccharide, but lacks the terminal trisaccharide.

Protein content of the purified LOS is and nucleic acid content less than 1% (A260 absorbance plus agarose gel electrophoresis).

Quality control documentation of each lot is provided with shipment.