Pro-Lyse™ Cell Lysis Buffers

10 minute Protein Extraction
Fast & Soluble Cell Lysis Protocol

*Pro-Lyse™ Yeast Lysis Buffer

Catalog # 970-510
Volume: 100 ml

Pro-Lyse™ Bacterial Cell Lysis Buffer

Catalog # 970-110
Volume: 100 ml

Pro-Lyse™ Direct-X Cell Lysis Buffer

Catalog # 970-710
Volume: 10 ml

*Pro-Lyse™ Detergent Free Mammalian Cell Lysis Buffer

Catalog # 980-310
Volume: 100 ml

*Pro-Lyse™ Detergent Plus Mammalian Cell Lysis Buffer

Catalog # 970-310
Volume: 100 ml

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Pro-Lyse™ Lysis Buffers are proprietary cell lysis reagents designed for gentle lysis of bacterial pellets, bacterial liquid cultures, mammalian cell culture, & yeast without the need for bead beater, sonicator, or French press mechanical disruption. While compatible with enzymes such as lysozyme, Pro-Lyse™ Lysis Buffers extract protein without enzymatic digestion. Pro-Lyse™ Lysis Buffers have been formulated to allow efficient cell lysis & extraction of soluble protein under non-denaturing conditions with reduced levels of detergent (>2 fold lower detergent than other buffers)

Pro-Lyse™ Lysis Buffers have been tested in a variety of E. coli, mammalian, & yeast expression systems.* Pro-Lyse™ Bacterial Cell Lysis Buffer can be added directly to frozen culture pellets. While Pro-Lyse™ Direct-X can be added directly to the bacterial culture without the need for cell pelleting prior to cell lysis. A brief 10 minute incubation in Pro-Lyse™ disrupts the cells and releases non-denatured, soluble proteins. Proteins can be directly screened or purified from the resulting extract.

Standard protocols used to purify histidine-tagged proteins as well as other fusion constructs including GST, maltose-binding protein, etc are compatible with Pro-Lyse™. In addition, intact non-fused recombinant proteins have been successfully purified from lysates by ion exchange chromatography.

Pro-Lyse™ Cell Lysis Buffers are compatible with the addition of constituents such as protease inhibitors, salts, chelating agents, reducing agents such as β-mercaptoethanol or DTT, etc. Although not necessary, lysozyme may also be used for initial cell lysis. DNase I can be used to reduce viscosity from genomic DNA release, if required.

*contact to inquire about your specific expression system

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